Biuret assay and lowry assay is a method used to determine the concentration of protein. SlideShare is one of the most popular websites to share presentations online and you can use it to showcase your PPT presentations or demonstrate your expertise using PowerPoint presentations that are converted to an online slideshow. Experiment (2). The Best PowerPoint SlideShare Presentation Examples (+Killer Tips) There's nothing like a good example to illustrate a point. Determination of Carbohydrate by Anthrone Method Aim: To estimate the amount of carbohydrate present in the given sample by using the Anthrone method. Enzyme Assay. In the Chrome browser, your SlideShare presentation download appears at the bottom of the screen: In Chrome, your SlideShare download is at the bottom of the browser window.
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The intensity of color is proportional to the protein content of the sample (Owusu-Apenten, 2002).

The Lowry method is best used with protein concentrations of 0.01-1.0 mg/mL and is based on the reaction of Cu+, produced by the oxidation of peptide bonds,with Folin-Ciocalteu reagent. This resource is designed for UK teachers. Updated: Sep 18, 2013. pptx, 606 KB. Since Linkedin acquired SlideShare for more than $119 millions in 2012, it is being integrated in the most popular professional network. this is our north american website. Lowry Powerpoint with significant paintings from his career accompanied by brief notes and questions.

Biuret test detect the presence of peptide bond. BIOCHEMISTRY LAB Title: Total Protein Estimation by Lowry Method Dr. Abhay Khandagle Head, Postgraduate Department and Research Centre in Zoology Prof. Ramkrishna More Arts, Commerce & Science College INDIA PDEA’s Prof.Ramkrishna More College, Akurdi, Pune (Affiliated to SPPU-PUNE); Mob.-+91 9370333535ajkhandagle@gmail.com 1 Lowry Method The Lowry method combines the biuret reagent with another reagent (the Folin-Ciocalteau phenol reagent) which reacts with tyrosine and tryptophan residues in proteins. our products can be a little different in each country.

Created: Jun 2, 2011. As the cells are removed from the living (in vivo) environment and subjected to experimental manipulations […] This gives a bluish color which can be read somewhere between 500 - 750 nm depending on the sensitivity required. Quantitative estimation of proteins by Lowry assay: Principle: In the past this has been the most commonly used method for determining protein concentration, although it is tending to be replaced by the more sensitive methods described below.

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